Figure 8

SK3 immunoreactivity in the striatum 3 days after an ischemic stroke. A. The ischemic lesion (scale bar, 500 μm) shows increased staining with the microglia/macrophage marker, 'ionized calcium-binding adapter-1' (Iba-1; rabbit polyclonal, 1:1000; Cy3-conjugated donkey anti-rabbit secondary, 1:400). The high-magnification image from the core (inset; scale bar, 20 μm) shows examples of activated microglia/macrophages, co-labeled with the nuclear stain, DAPI. B, C. Sections from the edge and centre of the lesion were co-labeled with tomato lectin and anti-SK3 (as in Figure 6). Scale bars: 100 μm for the main images; 20 μm for the inset. In panel B, note the unlabeled white matter tracts at the edge of the lesion (asterisks) surrounded by areas of diffuse SK3 staining. In panel C, almost every activated microglia/macrophage (examples marked with arrows) is co-labeled with SK3 and tomato lectin. Note the intense surface SK3 labeling of activated microglia/macrophages (inset).