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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Hemin inhibits NO production by IL-1β-stimulated human astrocytes through induction of heme oxygenase-1 and reduction of p38 MAPK activation

Figure 5

Effects of HO-1 overexpression. Human astrocyte cultures were transfected with 1 μg pLEX expression vector containing blank (pMock) or human HO-1 (pHO-1) sequences under a CMV promoter in Fugene 6 reagent for 72 h prior to IL-1β exposure for 72 h followed by protein collection with M-PER. Equal amount of proteins were loaded onto 12% (for HO-1) or 7.5% (for iNOS) acrylamide/bis gel for electrophoresis, transblotted onto nitrocellulose membrane and probed with mouse anti-HO-1 or rabbit anti-iNOS or anti-β-actin (as internal control) antibodies. C; untreated control.

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Journal of Neuroinflammation

ISSN: 1742-2094