Figure 6

Astrocytes inhibit MPO release from neutrophils. A. FACS-sorted resting or LPS-activated neutrophils were co-cultured with resting or poly I:C-primed astrocytes at a ratio of 1:1 for 6 hr. Semi-quantification of MPO shows fold change of MPO compared with resting neutrophils alone. White bar, neutrophils alone; grey bar, neutrophils co-cultured with resting astrocytes; black bar, neutrophils co-cultured with primed astrocytes (*p < 0.05, vs resting neutrophils alone; **p < 0.01, vs resting neutrophils alone; ++p < 0.01, vs activated neutrophils alone. n = 6/bar). B. MPO release by LPS-activated neutrophils was determined in a transwell plate following the same procedure as above. Resting neutrophils were as negative control (**p < 0.01, vs resting neutrophils alone; ++p < 0.01, vs activated neutrophils alone. n = 4/bar). C. Resting or LPS-activated neutrophils were incubated in the presence or absence of equal volume of astrocyte-conditioned medium followed by semi-quantification of MPO release. White bar, neutrophils alone; black bar, with astrocyte-conditioned medium. (**p < 0.01, vs resting neutrophils alone; ++p < 0.01, vs activated neutrophils alone. n = 5/bar).