Figure 5

Subchronic stress increases EAE-induced MMP-9 expression. A Again EAE and stress induced an increase in plasma corticosterone but expression of GR in spinal cord measured by western blot revealed that the EAE+S group showed lower levels, indicating a failure in HPA regulation (B). C MMP-9 expression in spinal cord by western blot showed an opposite pattern to that at day 21, with higher levels in the EAE+S group than in the EAE group. β-Actin expression was used as an internal control and densitometric analysis of the band is shown below. *p < 0,05 vs EAE;.# p < 0,05 vs Control, one way ANOVA test followed by a Newman-Keuls.