Figure 3
From: CCL2 modulates cytokine production in cultured mouse astrocytes
IL-6 production by IL-1β-stimulated astrocytes is concentration-dependent and enhanced in Ccl2 -/- cells. IL-6 levels were measured in astrocyte supernatant following stimulation with IL-1β at various concentrations (a) and time periods (b-d). At 1 ng/ml IL-1β, wild-type cells produced significantly more IL-6 compared to Ccl2-/- astrocytes from 2-24 h exposure, albeit at low levels. In contrast, at 10 and 50 ng/ml IL-1β, Ccl2-/- astrocytes produced significantly higher levels of IL-6 across the time course. Stars annotate p-values representing differences between strains from post-hoc analysis (*p < 0.05; **p < 0.01; ***p < 0.001), whilst the hash symbol designates differences between stimulation times/concentrations (# p < 0.001). Data represent mean + sem of three separate experiments assayed in duplicate. Spnt = supernatant.