Figure 7

Expression of CD11b, CD68, and MHC class II in spinal cord and ventral nerve root. Expression of CD11b, CD68, and MHC II in adjacent sections of ventral spinal cord and ventral nerve root of hmSOD1 rats. (A-C) In pre-clinical stage ventral horn, a cluster of CD11b-labeled microglia (A) in the ventral horn (VH, above dashed line) does not co-localize with CD68 (B) or MHC II (C). (D-F) In the ventral horn after clinical onset, CD11b expression (D) is more widespread and both CD68 (E) and MHC II (F) expression are detected. (G-I) In ventral nerve root after clinical onset, both CD11b- (G) and CD68-labeled (H) cells are increased, but MHC II-labeled cells (I) remain sparse. Scale bar in panel A represents 200 μm for all panels.