Figure 3

Adhesion of human and mouse DCs to myelin. (A) Adhesion of human DCs (8 healthy donors) to human myelin. Values were calculated as the fold change in adhesion on myelin compared to adhesion of the same cells to plastic control (plastic = 1). (B) Human immature and mature DC (grouped) NgR1 and NgR2 mRNA expression (expressed as 1/delta Ct, 1/ΔCt) correlate with adhesion to a myelin substrate (at 100 μg/ml), expressed as the fold increase in adhesion on myelin compared to plastic. NgR1, Spearman r = -0.7818, P = 0.0105. NgR2, Spearman r = -0.7697, P = 0.0126. (C) Adhesion of human DCs (3 donors) to myelin, Nogo-66-His, MAG-Fc, MBP (all at 10 μg/ml), IgG (15 μg/ml), expressed as fold increase in adhesion compared to on plastic. (D) Adhesion of mouse WT and Ngr1/2-/- (KO) DCs (9 mice of each genotype) to mouse myelin (10 μg/ml), expressed as fold increase in adhesion to myelin compared to plastic. Bars represent mean with SEM of the fold change in adhesion on myelin compared to adhesion of the same cells to plastic (plastic = 1). *P < 0.05, **P < 0.01.