Table 1 Primers used in quantitative real time PCR.
Target Gene | Accession | Primer forward (5→3') | Primer reverse (5→3') |
|---|---|---|---|
NOS2 | NM_010927.3 | ggCAgCCTgTgAgACCTTTg | gCATTggAAgTgAAgCgTTTC |
IL1β | NM_008361.3 | TggTgTgTgACgTTCCCATTA | CAgCACgAggCTTTTTTgTTg |
IL6 | NM_031168.1 | CCAgTTTggTAgCATCCATC | CCgCAgAggAgACTTCACAg |
TNFα | NM_013693.2 | TgATCCgCgACgTggAA | ACCgCCTggAgTTCTggAA |
TGFβ1 | NM_011577.1 | TgCgCTTgCAgAgATTAAAA | AgCCCTgTATTCCgTCTCCT |
IL4 | NM_021283, 2 | CgAggTCACAggAgAAgggA | AAgCCCTACAgACgAgCTCACT |
Actin | NM_007393.3 | CAACgAgCggTTCCgATg | gCCACAggATTCCATACCCA |
Rn18s | NR_003286.2 | gTAACCCgTTgAACCCCATT | CCATCCAATCggTAgTAgCg |