Figure 5
From: Apolipoprotein E expression is elevated by interleukin 1 and other interleukin 1-induced factors
Potential for indirect effects of IL-1β. A: Primary cortical neurons were treated for 20 h with the indicated concentrations of IL-1β, and glutamate levels were assessed in the medium (*p < 0.05). B: Primary cortical neurons were treated for 20 h with the indicated concentrations of IL-1β, and sAPP levels were assessed in concentrated aliquots of the culture medium by western blot; *p < 0.05. C: Primary cortical neurons were treated for 20 h with glutamate (50 μM) and Aβ1-42 (10 μM) alone, or with a combination of the two; immunofluorescence was performed for ApoE (green) and βAPP (red). D: The bar graph shows integrated fluorescence intensity quantified for the ApoE signal in the cultures shown in panel C; n = 3 cultures for each treatment (*p < 0.05 compared to the untreated sample).