Figure 7

The effect of LY294002 on TLR3/4- or IL-1/IFNγ-induced microglial inflammatory gene expression. Microglial cells were pre-treated with LY294002 for 1 h and then stimulated with LPS (100 ng/ml), PIC (10 μg/ml), or IL-1β and IFNγ (10 ng/ml) for 16 h. Q-PCR (left column: A, C and E) and ELISA (right column: B and D) were performed as described above. All values were normalized to those obtained in cultures stimulated with LPS, PIC or IL-1/IFNγ without LY294002 (= 1). At least 3 cases were analyzed for each experiment, and statistics were performed using t-test comparing values with vs. without LY294002.