Figure 1

CBD prevented LPS-induced vasodilation. (A) Representative 60 μm Z-stacks of subpial vessels in the mouse brain reflect the increase in blood vessel diameter after LPS administration (t = 0, left; t = 120 min, right). X-Y labels represent diameter in micrometers. Vascular tree is evidenced by i.v. administration of 70000 MW Texas red-conjugated dextrane and endothelial and microglial cells were labelled with topical fluorescein-conjugated Griffonia simplicifolia (green). (B) Treatment with CBD (triangles) significantly decreased the LPS-induced changes (black circles) in venules (left) and arterioles (right), as compared to vehicle treated animals (open circles). Error bars represent mean ± SEM of 7-8 experiments. ^†p < 0.05 by 2-way ANOVA. Scale bar: 250 μm.