Figure 3

Tat induces TNFα, IL-6, iNOS and SERT expression in OHSCs in a time-dependent manner. Hippocampal slices were treated with Tat (40 ng/slice) and tissue and media were collected at various times. Average Ct values at 2, 6 and 12 h were, respectively, for TNFα: 18.24 ± 0.76, 20.42 ± 0.75, 23.32 ± 1.01; IL-6: 21.08 ± 0.19, 19.88 ± 0.03, 21.98 ± 0.31; iNOS: 28.47 ± 1.91, 19.92 ± 0.14, 19.08 ± 0.25; SERT: 32.42 ± 0.28, 27.91 ± 0.02, 27.50 ± 0.18. Levels of TNFα and IL-6 (pg/ml) were measured in the supernatant by ELISA. Data represent the mean ± SEM (n = 3 in each group). Bars labeled with different letters (a, b, c or d) are significantly different from each other at p < 0.05.