Figure 1

IFN-γ induces MHC class II expression on WT and GFAP ^-/- Vim ^-/- deficient mouse astrocytes. Cell-surface expression of MHC class II molecules on WT (left) and GFAP ^-/- Vim ^-/- (right) astrocytes was analyzed by flow cytometry in the absence (Ctrl.) and 48 h after the addition of 600 U/ml IFN-γ (+IFN-γ). Note that in WT cells, the fraction of low fluorescence intensity MHC class II positive cells upon IFN-γ treatment appears to be higher than in IFN-γ treated GFAP ^-/- Vim ^-/- astrocytes. Dotted lines indicate isotype controls; solid lines indicate anti-Alexa Fluor⁴⁸⁸-MHC class II antibodies. The results are representatives of four independent experiments.