Figure 4

MHC class II molecules are expressed in late endosomes/lysosomes in IFN-γ-activated WT and GFAP ^-/- Vim ^-/- mouse astrocytes. Fluorescence images of IFN-γ-activated WT (a) and GFAP ^-/- Vim ^-/- (b) astrocytes after immunostaining with antibodies against LAMP1 and MHC class II molecules. The two signals overlap to a great extent (overlay), indicating that IFN-γ induces expression and localization of MHC class II molecules in late endosomes/lysosomes in WT and GFAP ^-/- Vim ^-/- astrocytes. Lower panels (insets) show boxed regions at higher magnification. Scale bars: 10 μm; 2 μm (insets). Arrowheads point to typical structures expressing both signals.