The enteric bacterial metabolite propionic acid alters brain and plasma phospholipid molecular species: further development of a rodent model of autism spectrum disorders

Table 7 Changes (nanomole percent by weight composition) in rat brain phosphatidylserine molecular species following intraventricular infusion with propionic acid (PPA) and phosphate buffered saline (PBS)

Molecular weight	Molecular species	Diacyl species	PBS	PPA
760	D34:1	16:0/18:1	0.90 ± 0.10	1.10 ± 0.21
790	D36:0	18:0/18:0	1.59 ± 0.41	1.65 ± 0.11
788	D36:1	18:0/18:1	18.10 ± 0.53	21.62 ± 0.63*
786	D36:2	18:0/18:2	2.36 ± 0.34	2.55 ± 0.17
816	D38:1	18:0/20:1	1.00 ± 0.15	1.22 ± 0.19
810	D38:4	18:0/20:4	4.18 ± 0.07	4.46 ± 0.12
840	D40:3	18:0/22:3	1.19 ± 0.23	1.20 ± 0.17
838	D40:4	18:0/22:4	4.67 ± 0.17	3.63 ± 0.14*
836	D40:5	18:0/22:5	6.40 ± 0.30	6.91 ± 0.43
834	D40:6	18:0/22:6	58.39 ± 0.98	54.49 ± 1.05*
832	D40:7	18:1/22:6	1.22 ± 0.08	1.17 ± 0.12
Total%			100	100
∑ Saturates			1.59 ± 0.15	1.65 ± 0.11
∑ Monounsat			19.99 ± 0.39	23.93 ± 0.66*
∑ Polyunsat			78.41 ± 0.58	74.45 ± 0.55*

Values (nanomole percent by weight composition) represent means ± standard errors. Means in the same row accompanied by asterisks are significantly different between treatments at LSD = 0.05, n = 12 per treatment group. D represents diacyl species. Monounsat, monounsaturated fatty acids; PBS, phosphate buffered saline solution; polyunsat, polyunsaturated fatty acids; PPA, propionic acid. Phosphatidylserine molecular species were identified using neutral loss scan of 87 D in ESI negative mode. The lipid components in the table are arranged based on the molecular species composition.

ISSN: 1742-2094