Figure 4

The levels of pSAPK-JNK Thr-183/Tyr-185 were unchanged 54 h after inducing pMCAO. The total SAPK-JNK and pSAPK-JNK Thr-183/Tyr-185 was measured in western blots of ipsilateral cerebral cortex (A, B) and hippocampus (C, D) homogenates. The bar graphs show total levels of SAPK-JNK (A, C) and pSAPK-JNK Thr-183/Tyr-185 (B, D), and representative blots. The data represent the mean of three to five independent experiments and β-tubulin or β-actin were used as loading controls. In both the cerebral cortex (A, B) or hippocampus (C, D), pMCAO had no major effect on the levels of total SAPK-JNK (A, C) or pSAPK-JNK Thr-183/Tyr-185 (B, D). Post-pMCAO estradiol treatment produced a slight decrease in pSAPK-JNK Thr-183/Tyr-185 levels in the cerebral cortex (B), and a significantly decreased in the levels of pSAPK-JNK in the hippocampus, as compared with the SV group (D). Data are expressed as the mean ± SEM (n = 6 rats per group). IE, pMCAO-estradiol; IV, pMCAO-vehicle; SE, Sham-estradiol; SV, Sham-vehicle.