Figure 3

Effect of (−)-epigallocatechin gallate on lipopolysaccharide-induced adhesion molecule expression of endothelial cells. (A-F) Human cerebral microvascular endothelial cells/D3 (hCMEC/D3) were treated with 100 ng/ml of lipopolysaccharide (LPS) for the indicated time periods (3, 6, 24, 48 h) or with LPS at the indicated concentrations for 6 h. For pretreatment, cells were treated with (−)-epigallocatechin gallate (EGCG) (1, 5, and 25 μM) for 1 h prior to LPS treatment. The mRNA expression was determined by quantitative real time PCR. Data are expressed as mean ± SD of three different experiments (*P < 0.05, **P < 0.01, compared with no treatment control or LPS treatment only). (G) Cells were treated with 100 ng/ml of LPS for the indicated time periods and protein was extracted for western blotting of adhesion molecule expression. (H) hCMEC/D3 cells were treated with EGCG for 1 h prior to LPS treatment for 6 h. Protein was extracted for western blotting of adhesion molecule expression. Representative data from three independent experiments are shown.