Figure 7

Effect of hypothermia on ROS production in LPS-stimulated microglial cells. BV-2 microglial cells (5 x 10⁴ cells per well in a 96-well plate) were treated with LPS (100 ng/ml) for six hours under normothermic (37 °C) or hypothermic (29 °C) conditions as indicated (A). Production of H₂O₂ was evaluated by Amplex Red assay (B). Results are the mean ± SD (n = 3). *P <0.05; significantly different from the LPS-treated group under normothermia. LPS, lipopolysaccharide; n, number; ROS, reactive oxygen species.