Figure 9

Effect of hypothermia on iNOS gene expression in hypoxia-stimulated microglial cells. BV-2 microglial cells or primary microglial cells (5 x 10⁵ per well in six well plates) were exposed to LPS (100 ng/ml) or hypoxia under hypothermia (29 °C) or normothermia (37 °C) as described (A). Total RNA was isolated at six hours after LPS or hypoxic stimulation followed by reoxygenation. The levels of iNOS mRNA were determined by conventional RT-PCR. The data was normalized to GAPDH (B, C). Results are the mean ± SD (n = 3). *P < 0.05; significantly different from the hypoxia-treated group under normothermia. iNOS, inducible NO synthase; LPS, lipopolysaccharide; n, number.