Figure 2

Syk is phosphorylated during phagocytosis of C3bi-opsonized myelin. (A) Total (T) and phospho (p) Syk levels were determined by immunoblot analysis in lysates of wild-type and control Con-Luc microglia before (0 min), and after 10 and 30 min of phagocytosis. (B) The ratio p/T at 10 and 30 min was calculated for each microglia type as percentage of p/T in respective non-phagocytosing phagocytes (i.e., at 0 min), which was defined as 100 %. Averages ± SEM of individual experiments, each performed in triplicates, are given. Significance of difference by one-way ANOVA and the Dunnet post test is *** p < 0.001. The immunoblot in (A) is from control Con-Luc microglia. Data in (B) were obtained from wild-type (squares) and Con-Luc (circles) microglia.