Figure 1From: Transgenic inhibition of astroglial NF-κB protects from optic nerve damage and retinal ganglion cell loss in experimental optic neuritis Histological analysis and quantification of myelinated axons in the optic nerve of mice with ON. (A) Clinical course of MOG(35–55)-induced EAE in WT and GFAP-IκBα-dn mice. EAE symptoms were scored daily for 60 days as described in Materials and Methods. Results are expressed as the daily mean clinical score ± SEM of 24 animals/group from three independent experiments;*P < 0.0001, Mann–Whitney test. (B) Histological analysis of PPD-stained, resin-embedded optic nerves of WT and GFAP-IκBα-dn mice under naïve conditions and at various times after induction of EAE. Scale bar = 10 μm. (C) Quantification of the total number of myelinated axons in PPD-stained cross-sections of the optic nerve using Stereoinvestigator software; ^P < 0.05 vs. WT naïve; *P < 0.05, one-way ANOVA, Tukey test. Ten mice/group were analyzed.Back to article page