Figure 7From: Small interfering RNA-mediated suppression of Ccl2 in Müller cells attenuates microglial recruitment and photoreceptor death following retinal degeneration Quantification of apoptosis following BCL by terminal dUTP nick end labeling (TUNEL) and activator protein (AP)-1 expression in retinas injected with chemokine (C-C motif) ligand (Ccl)2 small interfering (si)RNA. (A–D) Representative images from the superior mid-periphery show TUNEL (red) for nuclei situated predominantly in the ONL in the siRNA treatment groups following BCL exposure. E: Animals injected with Ccl2 siRNA found a marked decrease in the number of TUNEL-positive nuclei in the outer nuclear layer (ONL) (85.1, P < 0.05; ANOVA/Tukey’s test) compared with the Invivofectamine-only group and the scrambled siRNA control group after 24 hours of BCL (263.8 and 250.1 respectively). (F) Expression of AP-1 in the retina following BCL was reduced to 14.1-fold in retinas injected with Ccl2 siRNA (P < 0.05), compared with 20.5-fold and 24.9-fold reduction in the Invivofectamine-only group and the scrambled siRNA control group, respectively. Inivofectamine-only (n = 8), scrambled siRNA (n = 8), Ccl2 siRNA (n = 6) Error bars represent SEM. *Significant change at P <0.05 using ANOVA with Tukey’s post hoc test. NS, not significant.Back to article page