Protocol | Experiment | Time point | Group | Number | Total |
---|---|---|---|---|---|
Effect of EA pretreatment on α7nAChR expression | Western blot analysis Immunofluorescent staining | 24 h, 48 h, 72 h 72 h | Sham, Control, EA | n = 5 n = 3 | 44 |
Neuroprotective effect of PHA-543,613 pretreatment | Neurological evaluation and TTC staining | 72 h | MCAO, PHA-543,613+MCAO, Vehicle+MCAO | n = 10 | 30 |
Effect of α-BGT on EA pretreatment induced neuroprotection and HMGB1 release | Neurological evaluation and TTC staining | 72 h | Sham, MCAO, EA+MCAO, α-BGT+EA, Vehicle+EA, α-BGT+MCAO | n = 10 | 120 |
TUNEL staining | 24 h | n = 5 | |||
ELISA for HMGB1 level | 72 h | n = 5 |