Figure 1

The clinical course of hyperacute EAE in COAM-treated mice. (A) SJL/J mice were immunized subcutaneously with syngeneic SCH for induction of hyperacute EAE and treated i.p. at day 0 with 2 mg COAM. Mean disease scores were calculated for COAM-treated (n = 10, filled squares) or saline-treated (n = 10, open squares) animals. One significant data point is indicated by an asterisk at day 14. (B) When two injections with COAM were given on days 0 and 7 after immunization, significant reduction of disease scores (asterisks) were observed from day 12 onwards. The clinical course of hyperacute EAE in these mice is shown as mean daily group score ± SEM. Dead animals were scored grade 6 from the day of death until the end of the study. Black vertical arrows indicate time points of COAM administration. * P <0.05 for comparison with saline-treated group (Wilcoxon test). COAM, chlorite-oxidized oxyamylose; EAE, experimental autoimmune encephalomyelitis; SCH, spinal cord homogenate; SEM, standard error of the mean.