Figure 4

Blockage of corticosteroid-dependent IL - 6 induction by inhibition of NF - κB . BV-2 cells were pretreated with 250 nM of the NF-κB inhibitor Cay-10512 for 1 h, followed by incubation with 25 nM corticosterone, 50 nM 11-dehydrocorticosterone, or aldosterone for another 24 h. IL-6 protein was measured by ELISA. Results represent mean ± SD from three independent experiments. **P <0.01 and ***P <0.005.