Figure 7
From: Therapeutic targeting of Krüppel-like factor 4 abrogates microglial activation
Effects of honokiol on Krüppel-like factor 4 expression in lipopolysaccharide-treated primary microglia. Primary microglia isolated from P0-P2 BALB/c mice treated with 500 ng/mL of LPS and 10 μM of HNK were analyzed by immunocytochemistry. (A) Fluorescent images of untreated primary microglia treated with a sham mixture of 1X PBS and DMSO in a 1:1 ratio. (B) Primary microglia treated with LPS for 12 hours and (C) LPS-treated primary microglia co-treated with HNK for 12 hours. Images showing the staining for Klf4 (Alexa fluor 594; Rhodamine channel); DAPI (blue) and merged images (right panel). Scale bar: 20 μm.