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Table 2 Primary antibodies used for immunohistochemistry

From: IFN-gamma signaling in the central nervous system controls the course of experimental autoimmune encephalomyelitis independently of the localization and composition of inflammatory foci

Antigen

Antibody

Ly6G

FITC-conjugated rat anti-mouse Ly6G

BD Pharmingen (Cat#551460), 1:100

IBA1

Rabbit polyclonal anti-IBA1

Wako (Cat#019-19741), 1:1000

CD11b

Rat anti-mouse CD11b

BD Pharmingen (Cat#550282), 1:100

CD4

Rat anti-mouse CD4

BD Pharmingen (Cat# 550280), 1:100

CD45.1

PE-conjugated mouse anti-mouse CD45.1

eBioscience (Cat#12-0453-82), 1:100

CD45.2

PE-conjugated mouse anti-mouse CD45.2

BD Pharmingen (Cat# 560695), 1:100

CD45.2

FITC-conjugated mouse anti-mouse CD45.2

eBioscience (Cat#11-0454-85)

SMI32

Mouse anti-mouse NF-H

Covance (Cat# SMI-32R), 1:1000

STAT1

Rabbit polyclonal anti-human STAT1

Santa Cruz (Cat# SC592), 1:100

CD45R

PE-Texas Red rat anti-mouse CD45R/B220

BD Pharmingen (Cat# 551489), 1:100

IL4

Goat anti-mouse IL4

R&D (Cat# AF-404-NA)

MBP

Rat anti-bovine myelin basic protein

Novus biologicals (Cat# NB600-717)