Figure 5

Double labeling of primate bulbus oculi tissue sections with patient serum and a commercial antibody to anti-ITPR1. An overlay of the index patient’s IgG and a commercial antibody to ITPR1 was also observed outside the CNS and the intestine, e.g., in the eye bulb (A: ciliary muscle, B: retina with rod and cone processes), confirming the specificity of the patient antibody for ITPR1. Anti-ITPR1 reactivity is depicted in red (Alexa Fluor® 568), the patient antibody in green (Alexa Fluor® 488), and yellow indicates overlay of the two antibodies. Nuclei are shown in blue (DAPI).