Figure 3

Expression levels of total cerebral dopamine neurotrophic factor (CDNF) in the substantia nigra (SN). Graphs showing the fold change increase in the endogenous, rat (r)CDNF (A) and relative transgene human (h)CDNF (B) transcripts by qRT-PCR. Graphs showing the protein levels of rCDNF (C) and hCDNF (D) by ELISA. First 3 bars in the graphs represent controls and the rest represent the time course of p3xNBRE-hCDNF transfections. FT15 = at day 15 after p3xNBRE-EGFP transfection, ¼ = at 6 hours; 1 = at 24 hours; 3 = at day 3; 7 = at day 7; 15 = day 15, after p3xNBRE-hCDNF transfection in a 15-day lesion rats. (E) Representative merged confocal micrographs showing immunoreactivity to CDNF (green) and tyrosine hydroxylase (TH) (red). The headings refer to mesencephalon slices that were obtained from different rats, intact (INTACT), with 30-day 6-OHDA lesion (L30), and 15 days of lesion and 15 days of transfection (L15T15). The scale bars = 20 μm are common for all the micrographs. ns = not significant. **P < 0.01, ***P < 0.001 when compared with the intact condition. £ = P < 0.001 when compared with L30. One-way ANOVA with Newman-Keuls post hoc test. n = 4 different rats for each condition.