Figure 6

AMWAP prevents LPS-induced degradation of IRAK-1 and IκBα. Control and AMWAP-treated BV-2 microglia were incubated with 50 ng/ml LPS for 0, 1, 2, and 3 h, and cytosolic protein extracts were prepared. Immunoblot analysis of IRAK-1 (A) and IκBα (B) was carried out to determine the level of proteolytic degradation. In AMWAP-treated cells, both signaling molecules were protected from LPS-induced proteolysis. Resynthesized IκBα was detected after 2 and 3 h in control cells. Actin served as loading control.