Fig. 3
From: Altered morphological dynamics of activated microglia after induction of status epilepticus
Faster process motility of activated microglia towards a pipette containing 2Me-ADP. a Maximal intensity projection of two-photon images at different time points after the insertion (at t = 0) of a pipette containing 2Me-ADP (100 μM) in slice from control (left column) or kainate-treated (right column) animals. At t = 25 min (right column), the processes of activated microglia have reached the pipette (bottom images), whereas those of microglia from control mice reached their target only after 45 min (inset picture). Scale bar, 15 μm. b Cumulative probability of all elongation movements by single process tips measured in control (black) and KA-injected (red) animals (p < 0.001, KS test). c Velocity measured with two different methods in control (black) and KA-injected (red) animals. Global velocity was assessed measuring the fluorescence in concentric rings around the pipette tip. Process elongation velocity was evaluated considering the median of average elongation tip velocity of several processes in the experiment. Both methods revealed a higher velocity in KA-injected animals compared to control (p < 0.01, t test)