Fig. 7
Effect of LPS and indomethacin (Indo) on TEER and 14C-sucrose permeability in monolayers of BEC monocultures (EOO, n = 10), BEC + astrocyte co-cultures (EOA, n = 5), BEC + pericyte co-cultures (EPO, n = 5), and BEC + pericyte + astrocyte tri-cultures (EPA, n = 10). Panel a shows that in monocultures of BECs, LPS increased permeability to sucrose and that indomethacin partially blocked this effect. Panel b shows that LPS increased permeability of monolayer monocultures of BEC as measured by TEER and that indomethacin had no effect on LPS-induced permeability. Co-cultures of BECs with astrocytes (panels c and d) or pericytes (panels e and f) or tri-cultures of BECs with pericytes and astrocytes (panels g and h) did not produce results substantially different from those obtained with monocultures of BECs. Y-axis is %Control of Pe in units of microliters per minute per square centimeter. *p < 0.05, ***p < 0.001; without bar compares to respective 0 value; with bar compares LPS 10 μg/ml without indomethacin to LPS 10 μg/ml with indomethacin