Table 1 Primer pairs used in this study
Gene target | Primer name | Sequence |
|---|---|---|
GAPDH | GAPDH For | 5′-CAAGGTCATCCATGACAACTTTG-3′ |
GAPDH Rev | 5′-GGGCCATCCACAGTCTTCTG-3′ | |
IL-1ß | IL-1ß For | 5′-TGTGGCAGCTACCTATGTCT-3′ |
IL-1ß Rev | 5′-GGGAACATCACACACTAGCA-3′ | |
TNF-α | TNF-a For | 5′-CATCTTCTCAAAACTCGAGTGACAA-3′ |
TNF-a Rev | 5′-TGGGAGTAGATAAGGTACAGCCC-3′ | |
IL-6 | IL-6 For | 5′-TCACAGAAGGAGTGGCTAAGG-3′ |
IL-6 Rev | 5′-GCTTAGGCATAGCACACTAGG-3′ | |
TLR2 | TLR2 For | 5′-TCCATGTCCTGGTTGACTGG-3′ |
TLR2 Rev | 5′-AGGAGAAGGGCACAGCAGAC-3′ | |
TLR4 | TLR4 For | 5′-GATTGCTCAGACATGGCAGTTTC-3′ |
TLR4 Rev | 5′-CACTCGAGGTAGGTGTTTCTGCTAA-3′ | |
TLR3 | TLR3 For | 5′-TGAAAACTACGGCGATGCAG-3′ |
TLR3 Rev | 5′-AGGCAGTTTTACTTCCCCGA-3′ |