Fig. 5

Systemic administration of LY303870, anakinra, and anti-NGF poorly reversed fracture-induced nociceptive responses at 16 weeks. Vehicle, LY303870, anakinra, or anti-NGF was administered between 15 and 16 weeks post fracture, and the rats were tested at 16 weeks post fracture. LY303870 (same treatment as Fig. 4) was injected for 7 days prior to testing, anakinra (100 mg/kg i.p.) was injected once at 1 h prior to testing, and anti-NGF (same treatment as Fig. 4) was injected once at 7 days prior to testing at 16 weeks post fracture. a Mechanical allodynia tested by von Frey fibers persisted to 16 weeks post fracture. LY303870, but not anakinra or anti-NGF, reversed fracture-induced allodynia at the 16-week time point. b Approximately 10 % unweighting remained at 16 weeks post fracture. Both LY303870 and anti-NGF reversed this remaining hindpaw unweighting. Data are expressed as mean values ± standard error (SE) and analyzed to compare fracture at 16 weeks versus non-fracture control cohorts and fracture with treatments versus fracture at 16 weeks cohorts using one-way ANOVA followed by Neuman-Keuls multiple comparison test. *P < 0.05, **P < 0.01, and ***P < 0.001 versus control rats; ^# P < 0.05, ^## P < 0.01, and ^### P < 0.001, fracture with treatments versus fracture at 16 weeks (control cohort, n = 10; FX- 4 WK, n = 8–10; FX- 4 WK + LY303870, n = 8–9; FX- 4 WK + anakinra, n = 6; FX- 4 WK + anti-NGF, n = 6–8)