Fig. 4
From: Cocaine-mediated induction of microglial activation involves the ER stress-TLR2 axis
Role of Oxidative-ER stress in cocaine-mediated up-regulation of TLR2 and microglial activation in BV2 cells. a, b Effect of pharmacological inhibitor of ER-stress 4-PBA on cocaine-mediated up-regulation of ATF4 and TLR2 proteins in BV2 cells. c Effect of 4-PBA on cocaine-mediated induction of TLR2, TNFα, IL-6, and MCP-1 mRNAs. Relative levels of TLR2, TNFα, IL-6, and MCP-1 mRNAs were analyzed by qPCR using 18S rRNA as an internal control. d Effects of 4-PBA on cocaine-mediated induction of TNFα and MCP-1 proteins assessed by ELISA. e 4-PBA did not ameliorate cocaine-mediated induction of ROS generation. f, g Effects of APO (NADPH oxidase inhibitor) and PBN (ROS scavenger) on cocaine-mediated up-regulation of ATF4 protein in BV2 cells. All data are presented as mean ± SD of three individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs control group. # p < 0.05, ## p < 0.01 vs cocaine group (Student’s t test)