Fig. 4

Microglia trajectories showed higher percentage of active migrating microglial cells in healthy brain slices. a Overlay of individual microglial cells trajectories, plotted after aligning their starting positions. Cells were tracked over a 6-h period. Units are in micrometers. Restrictive migration was defined as microglial cells migrating within 20 μm from their starting point (shaded area); active migration was defined as migration of more than 20 μm from their starting point. A1: 42 microglial cells from healthy brain slices; A2: 48 microglial from CP brain slices. b The quantitative study demonstrated higher “active migrating” microglia population in healthy rabbit brain slices. In the brain slices from healthy rabbits, more than half the microglia cells (52.4 %) showed active migration pattern, while 83.3 % of microglia from CP rabbit brain slices showed restrictive migration pattern