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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Neonatal inflammatory pain and systemic inflammatory responses as possible environmental factors in the development of autism spectrum disorder of juvenile rats

Fig. 4

Neonatal inflammatory pain decreased neurogenesis in the juvenile brain. Immunofluorescent double labeling for DCX and BrdU was performed to examine neurogenesis in P21 rats. a–d Double-labeled images of BrdU (red) and DCX (green) in the dentate gyrus of the control (a, b) or formalin (c, d) group. Nuclei were stained with Hoechst 33342 (blue). b, d 3-D confocal images of BrdU (red) and DCX (green) in the dentate gyrus of the control and formalin groups. Scale bars = 50 μm. e, f Summarized data of the total numbers of DCX-positive and DCX/BrdU-positive cells in three sections of each animal. There were fewer BrdU-positive (e) and DCX/BrdU-positive cells (f) in the formalin group compared to the control group. ***P < 0.001 vs. control; n = 6 per group

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Journal of Neuroinflammation

ISSN: 1742-2094