Fig. 1
From: The crucial role of Erk2 in demyelinating inflammation in the central nervous system
Phosphorylation of Erk1/2 during cuprizone-induced demyelination. a Progressive demyelination observed in the corpus callosum as shown by reduced luxol fast blue (LFB) staining. d, W days or weeks of cuprizone feeding. b Phosphorylation of Erk1 and Erk2 shown by protein analysis of the corpus callosum. Three independent experiments showed the same trend. The representative data is shown. c Phosphorylation of Erk1 and Erk2 shown by immunohistochemistry of the region. d At the fourth model week, when demyelination occurs, gliosis is observed as shown by increased immunoreactivity for GFAP and Iba-1. Lines in d and e indicate the boundaries of the corpus callosum; white, double-headed arrows indicate the area of the corpus callosum. e, f Multiple immunostaining indicates that a phosphorylated form of Erk1/2 becomes prominent and colocalizes with GFAP-positive cells (e, f arrowhead) but only occasionally with CC1- or Iba-1-positive cells. Cup cuprizone treatment duration; TO-PRO®-3, a nuclear stain; scale bars: a, c, and d, 100 μm; e and f, 50 μm