Table 4 Primers for mutagenesis
From: miR-124a and miR-155 enhance differentiation of regulatory T cells in patients with neuropathic pain
Primer | Sequence | Position |
|---|---|---|
SIRT mut 124a for | 5′-TATTTAAAAGCTTAGCCTGGATTAAAACTAGAGATCAACTTTCTCAGA-3′ | 1211–1217 |
SIRT mut 124a rev | 5′-GCTGAGAAAGTTGATCTCTAGTTTTAATCCAGGCTAAGCTTTTAAATA-3′ | |
SIRT mut 155_1 for | 5′-CAGGAATTGTTCCACCAGGGTTAGGAACTTTAGCATGTC-3′ | 36–42 |
SIRT mut 155_1 rev | 5′-GACATGCTAAAGTTCCTAACCCTGGTGGAACAATTCCTG-3′ | |
SIRT mut 155_2 for | 5′-TTGATCTTTTCCACAAGGGTTAAACTGCCAAAATGTG-3′ | 929–935 |
SIRT mut 155_2 rev | 5′-CACATTTTGGCAGTTTAACCCTTGTGGAAAAGATCAA-3′ | |
SIRT mut 155_3 for | 5′-GAAATTGCACAGTAAGGGTTTATTTTTCAGACCATT-3′ | 1408–1414 |
SIRT mut 155_3 rev | 5′-AATGGTCTGAAAAATAAACCCTTACTGTGCAATTTC-3′ |