Fig. 1

PARP deficiency diminishes leukocyte adhesion to and migration across the BBB and reduces BBB permeability. Representative images from videos of leukocytes labeled with Rhodamine 6G for adhesion (a) and migration (b). Measurement of labeled leukocytes after their adhesion to (c) or migration (d) across the endothelium. e Measurement of BBB permeability in vivo by sodium-fluorescein accumulation in the brain in the presence or absence of LPS. Results are shown as mean adhesion or migration ± SEM (four animals per treatment group). **P < 0.01 indicates significance vs. TNFα or LPS-treated animals. Scale bar = 100 μm is shown in one micrograph, but it is valid for all micrographs shown