Fig. 1From: Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse modelAnalysis of BFP-MSC and IL13-MSC graft remodelling in the CNS of eGFP+ bone marrow chimaeras. a Overview of a BFP-MSC and IL13-MSC graft. Green: eGFP fluorescence from bone marrow-derived macrophages indicating infiltration at the graft. Red: GFAP staining indicates astrogliosis around the graft. Scale bars indicate 200Ā Ī¼m. b Detail of the BFP-MSC and IL13-MSC graft. White: TOPRO-3 marking all nuclei. Green: eGFP fluorescence from graft-infiltrating macrophages (arrows). eGFPā nuclei (arrowheads) are grafted MSC or (few) graft-infiltrating microglia. Scale bars indicate 50Ā Ī¼m. Insets show BFP fluorescence (blue) or no fluorescence (black) from grafted BFP-MSC or IL13-MSC (arrowheads). Green: eGFP fluorescence from graft-infiltrating macrophages (arrows). Scale bars indicate 20Ā Ī¼m. c Overview of a BFP-MSC and IL13-MSC graft. Green: eGFP fluorescence from graft-infiltrating macrophages. Red: Iba1 staining marks both eGFPā microglia and eGFP+ macrophages. Scale bars indicate 200Ā Ī¼m. Details of the BFP-MSC and IL13-MSC implant and border show Iba1+eGFP+ peripheral macrophages mainly within the implant (arrows) and Iba1+eGFPā microglia mainly within the border (arrowheads). Scale bars indicate 50Ā Ī¼m. d Overview of a BFP-MSC and IL13-MSC graft. Green: eGFP fluorescence from graft-infiltrating macrophages. Red: MHCII-expressing eGFPā brain-resident microglia and eGFP+ peripheral macrophages. Scale bars indicate 200Ā Ī¼m. Details of the BFP-MSC and IL13-MSC implant and border are provided, showing activated MHCII+eGFP+ macrophages (arrows) and activated MHCII+eGFPā microglia (arrowheads). Scale bars indicate 50Ā Ī¼m. e Cell densities of MHCII+eGFP+ BM-derived macrophages and MHCII+eGFPā microglia within the implant and border of BFP-MSC and IL13-MSC grafts. Values are given as meanā+āstandard deviation. Significant differences (p value ā¤0.05) are indicated with an asterisk. f Overview of a BFP-MSC and IL13-MSC graft. Green: eGFP fluorescence from graft-infiltrating macrophages. Red: Arg1-expressing eGFPā microglia and eGFP+ macrophages. Scale bars indicate 200Ā Ī¼m. Details of the BFP-MSC and IL13-MSC implant and border are provided, showing alternatively activated Arg1+eGFP+ macrophages (arrows) and Arg1+eGFPā microglia (arrowheads). Scale bars indicate 50Ā Ī¼m. g Cell densities of Arg1+eGFP+ BM-derived macrophages and Arg1+eGFPā microglia within the implant and border of BFP-MSC and IL13-MSC grafts. Values are given as meanā+āstandard deviation. Significant differences (p value <0.05) are indicated with an asterisk. h Overview of a BFP-MSC and IL13-MSC graft. Green: eGFP fluorescence from graft-infiltrating macrophages. Red: Ym1-expressing eGFPā microglia and eGFP+ macrophages. Scale bars indicate 200Ā Ī¼m. Details of the BFP-MSC and IL13-MSC implant and border are provided, showing Ym1+eGFP+ BM-derived macrophages (arrows) and Ym1+eGFPā microglia (arrowheads). Scale bars indicate 50Ā Ī¼mBack to article page