Fig. 5

Representative images illustrating multiple phenotypes of microglia and macrophages in the control and experimental groups. Representative overview images (odd rows, scale bar indicates 200 μm) of the splenium of the corpus callosum (CC) in control mice (first column), CPZ-treated mice (second column) and CPZ-treated mice with BFP-MSC grafts (third column) or IL13-MSC grafts (fourth column). Representative detail images (even rows, scale bar indicates 50 μm) from the BFP-MSC or IL13-MSC graft site or from the corresponding areas in healthy control or CPZ control mice. First and second rows: in green direct eGFP fluorescence from CX3CR1^+/eGFP brain-resident microglia and in red direct RFP fluorescence from peripheral CCR2^+/RFP macrophages. Third and fourth rows: additionally in blue immunofluorescent staining for F4/80 marking activated eGFP⁺ brain-resident microglia and RFP⁺ peripheral macrophages. Fifth and sixth rows: additionally in blue immunofluorescent staining for MHCII marking activated eGFP⁺ brain-resident microglia and RFP⁺ peripheral macrophages. Seventh and eighth rows: additionally in blue immunofluorescent staining for Arg1 marking alternatively activated eGFP⁺ brain-resident microglia and RFP⁺ peripheral macrophages