Fig. 7

Acute microglial response to LPS. a Eleven-month-old female mice underwent PBS (1 μL) or LPS (0.1 μg) injection (n = 7 and n = 8 respectively for WT mice and n = 6 and n = 12 respectively for the Tollip KO mice). mRNA amounts in midbrain extracts were normalized by β-actin and GAPDH. b Representative CD68 and Iba1 immunostainings in non-injected (left) and LPS (0.1 μg)-injected side (right) in WT and Tollip KO 15-month-old male mice. Magnification = ×40. Scale bar = 30 μm. c Quantification of CD68 and Iba1-positive cells, performed on two fields in the injected area, on three sections per animal (n = 4 in WT and 5 mice in KO group). Results are represented as box and whiskers that show median, min., and max. values, 25th and 75th percentiles. Plots represent individual values and means are represented as a “+.” ^# P < 0.05; ^## P < 0.01; ^### P < 0.001 versus non-injected side and ^* P < 0.05; ^*** P < 0.001 versus WT mice. Two-way ANOVA followed by a Tukey’s post hoc test