Table 1 List of primer sequences for mouse PCR
Primer sequence 5′→3′ | Accession no. | Amplicon length (bp) | Region | |
SYBR green | ||||
TNF-α (Tnfa) | F: GGGGCCACCACGCTCTTCTGTC | NM_013693 | 155 | Exon 1 |
R: TGGGCTACGGGCTTGTCACTCG | Exon 3 | |||
Glucose-6P-dehydrogenase (G6pd) | F: CACAGTGGACGACATCCGAAA | NM_008062.2 | 103 | Exon 4 |
R: AGCTACATAGGAATTACGGGCAA | Exon 5 | |||
iNOS (Nos2) | F: CAGCTGGGCTGTACAAACCTT | NM_010927 | 95 | Exon 17 |
R: CATTGGAAGTGAAGCGTTTCG | Exon 18 | |||
VEGFA (Vegfa) | F: ATCTTCAAGCCGTCCTGTGTGC | NM_001025257.3 | 223 | Exon 3 |
R: TTGGCTTGTCACATTTTTCTGG | Exon 5/6 | |||
rpl14 | F: GGCTTTAGTGGATGGACCCT | NM_025974 | 143 | Exon 3 |
R: ATTGATATCCGCCTTCTCCC | Exon 4 | |||
ID Assay | Accession no. | Amplicon length (bp) | Exon boundary | |
Taqman probes | ||||
EP1 (Ptger1) | Mm00443098_g1 | NM_013641.2 | 85 | Exon 2–3 |
EP2 (Ptger2) | Mm00436051_m1 | NM_008964.4 | 73 | Exon 1–2 |
EP3 (Ptger3) | Mm01316856_m1 | NM_011196.2 | 79 | Exon 1–2 |
EP4 (Ptger4) | Mm00436053_m1 | NM_001136079.2 | 70 | Exon 2–3 |
NM_008965.2 | ||||
NOX2 (Cybb) | Mm01287743_m1 | NM_007807.5 | 63 | Exon 12–13 |
gapdh | Mm99999915_g1 | NM_001289726.1 | 107 | Exon 2–3 |
NM_008084.3 | ||||
hprt1 | Mm00446968_m1 | NM_013556.2 | 65 | Exon 6–7 |