Fig. 3

WWL70 suppressed COX-2 expression. Western blots of BV2 cell lysates with different treatment protocols, showing COX-2 expression with β-actin controls. The blots shown here are representative of three independent experiments. a BV2 cells were incubated with synthetic CB receptor agonist, WIN55,212-2, or 2-AG for 15 min, before addition of LPS (0.1 μg/ml) for 18 h. Single and double asterisks denote p < 0.05 and 0.01, respectively, compared to the LPS treatment alone (mean ± SD, n = 3). b BV2 cells were treated with AA or endocannabinoids at the indicated concentrations for 15 min followed by incubation with LPS for 18 h. Single, double, and triple asterisks denote p < 0.05, 0.01, and 0.001, respectively, when compared to the LPS treatment alone (mean ± SD, n = 3). c BV2 cells were treated with WWL70 for 15 min, followed by the addition of 2-AG for a further 15 min, before addition of LPS for 18 h. Relative COX-2 expression levels to β-actin were shown under each blot after normalizing with the LPS-treated conditions. Single asterisk denotes p < 0.05 (mean ± SD, n = 3). WIN refers to WIN55,212-2, and Noldn refers to 2-arachidonyl glyceryl ether