Fig. 6

ABHD6 siRNA knockdown did not reduce PGE₂ production. a qRT-PCR of ABHD6 transcript normalized to GAPDH, 24 h after transfection with ABHD6-specific siRNA. b Membrane fraction prepared from the transfected cells after 48 h transfection was reacted with the ABHD6-specific HT-01 probe for 30 min to detect active ABHD6 enzyme, followed by western blotting of calnexin as a loading control. c Cell cultures after 48 h transfection were replaced with fresh medium with LPS (0.1 μg/ml), WWL70 (10 μM), or 2-AG (10 μM). The culture medium was collected after 18 h incubation and employed PGE₂ EIA. Data are analyzed with two-way ANOVA and represented as mean ± SD (n = 3). Double or triple asterisks denote p < 0.01 or 0.001, respectively