Fig. 7
WWL70 inhibited PGE2 production in LPS-activated BV2 cells. Cells were treated with LPS (0.1 μg/ml) for 18 h to activate PGE2 biosynthesis before replacement with fresh DMEM containing (a) 10 μM 2-AG or AA and WWL70 (1 or 10 μM) or (b) 10 μM 2-AG or AA and WWL70 (10 μM) or KT182 (100 nM). The culture medium after 30 min incubation was employed PGE2 EIA. Using the microsome fraction, mPGES-1, mPGES-2, and β-actin protein expression after 30 min incubation with different treatment conditions was examined by western blot as shown below the EIA results. Data are represented as mean ± SD (n = 3). Single and triple asterisks denote p < 0.05 and 0.001, respectively