Fig. 4

Mitochondrial impairments in the EMD mice cerebral cortex. a OXPHOS subunits in EMD and control mice. Representative OXPHOS bands of cortical samples are shown (left panels). Protein levels of representative subunits of each of the five OXPHOS complexes were quantified (right panels). b Mitochondrial mass assessment performed in different regions from cortical sections from all EMD and control mice. Quantitative analysis of fluorescence targeting the ATP synthase β subunit revealed higher mitochondrial mass in EMD mice compared to control mice (left panels). c Representative western blot is shown for mitofusin 2 (Mfn2). Right panel shows increased protein levels of Mfn2 in EMD mice compared to control mice. Data are presented as mean ± SEM. *P < 0.05; Student’s t test.