Fig. 8

Recruitment of T lymphocytes into the choroid plexus (CP) is a requirement for the meningeal macrophage activation. a, b Quantitative analyses of the number of CD4⁺ T cells in the spleen (a) and CPs (b) of the PBS-Reg group, BCG-Reg group, PBS-Enr group, and the BCG-Enr group. c The graphs show expression analyses of the ICAM-1 gene, VCAM-1 gene, IFN-γ gene, TNF-a gene, and IL-6 gene in the CP of the BCG-Enr mice relative to the controls (PBS-Reg mice) at the indicated times (PD3, PD7, PD14, and PD21). d, e Representative micrographs (d) and analyses (e) of the CPs stained for ICAM-1 (red) and with nuclear staining for Hoechst (blue) from the PBS-Reg group, BCG-Enr group, and the PBS-Reg group treated with IFN-γ. f Quantitative analyses by RT-PCR of IL-10 mRNA levels in the CPs from PBS Reg group, BCG Reg group, and PBS Enr group treated with IgG or anti-TCR antibodies. g Confocal micrographs of the CPs stained for ICAM-1 (red) and cytokeratin-18 (green) from PBS Reg treated with IFN-γ group. *p < 0.05, **p < 0.01, ***p < 0.001 between the indicated groups by two-way ANOVA, LSD post hoc test, and Student’s t test (n = 6 per group). Scale bars 100 μm in b; 50 μm in d and in g. The data are presented as the means ± SEMs