Fig. 7
Reduced pro-inflammatory gene expression in C/EBPβ-deficient microglia in vivo and in vitro. Expression of C/EBPβ and five pro-inflammatory genes (Tnfa, Il23a, Csf3, Ptges and Cybb) was analyzed by qRT-PCR in microglia isolated from the brains of C/EBPβfl/fl and LysMCre-C/EBPβfl/fl mice treated i.p. with vehicle (C) or LPS (4 mg/kg) for 16 h (n = 4 mice/condition). Insets show the expression of the same genes in primary microglial cultures from both genotypes treated with vehicle (C), LPS (100 ng/mL) or LPS + IFNγ (1 ng/mL) for 6 h (n = 4 independent experiments). In all graphs, data are shown as mean + SEM. The asterisk shows the significance of the treatment effect, *p < 0.05; **p < 0.01; ***p < 0.001 compared with the respective control conditions. The number sign shows the significance of the genotype effect; #p < 0.05; ##p < 0.01; ###p < 0.001 compared with the respective C/EBPβfl/fl condition